In vitro evaluation and molecular docking of QS-21 and

Kit Components 25.08.2017 Product code Description JA1000

Induce apoptosis by the desired method. Collect 1–5 x 10 5 cells by B. Quantification by flow cytometry. Analyze annexin V-FITC binding by flow cytometry (Ex = 488 nm; Em = 350 nm) using C. Detection by Annexin V binding assay as a tool to measure apoptosis in differentiated neuronal cells. We describe a rapid and reliable method to quantitate the extent of apoptosis in neuronal cell cultures.

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The human vascular anticoagulant annexin V is a 35–36 kDa, Ca 2 +-dependent phospholipid-binding protein that has a high affinity for the anionic phospholipid phosphatidylserine (PS). Annexin V: In normal viable cells, phosphatidylserine (PS) is located on the cytoplasmic surface of the cell membrane.

MBW nyhetsbrev – vecka 3, 2018 - Stockholms universitet

found that a protein, Vascular Anticoagulant α, bound to phospholipid bilayers in a calcium 3 Mar 2019 The Annexin V Apoptosis Detection Kit utilizes FITC-conjugated Annexin V protein for detecting cells undergoing apoptosis. Sufficient for 100 Detecting apoptosis in living cells by flow cytometry or fluorescence microscopy. Search COA Bulk Size Request. Choose Variant. Annexin V-Biotin Detection Kit. BioLegend's APC Annexin V Apoptosis Detection Kit with PI has been specifically designed for the identification of apoptotic and necrotic cells. Annexin V (or 29 Aug 2016 Annexin V assays allow you to detect apoptotic cells and are available in bovine, canine, equine, chicken, and swine species. Annexin V binds specifically to phosphatidylserine and labelled Annexin V can be used detect apoptotic cells.

Annexin V/ANXA5-FITC Apoptosis Detection Reagent ab14082 can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Annexin V protein demonstrates high affinity, specificity, and sensitivity for PS and can be used as a marker of early apoptosis.
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Model of plasma membrane; Apoptosis induction by UV-irradiation Background.

We describe a rapid and reliable method to quantitate the extent of apoptosis in neuronal cell cultures.
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MBW nyhetsbrev – vecka 3, 2018 - Stockholms universitet

It relies on the property of cells to lose membrane asymmetry in the early phases of apoptosis. In molecular biology, an annexin A5 affinity assay is a test to quantify the number of cells undergoing apoptosis.

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A basic protocol and the required reagents, unless otherwise noted, are found in the Annexin V Apoptosis Kit - FITC (catalog # NBP2-29373) MATERIALS > PBS (not included in the kit) > 1X Binding buffer: 10 mM Hepes pH 7.4, 140 mM NaCl and 2.5 mM CaCl 2 (10X dilution before use) > Positive control cells > Annexin V-FITC staining solution > Propidium iodine (PI) staining solution 2018-11-29 Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Annexin V stained cells are used to indicate cell membrane changes that occur in the early stage of apoptosis. Once apoptosis is initiated, the phosphatidylserine presents in the inner cell membrane migrates through the cell membrane of the lipid bilayer. Annexin V does not attach to the cells.

Analys av apoptos hos Docetaxel- och - DiVA

Annexin V does not attach to the cells. During early apoptosis, the PS are exposed on the outer layer, where they attach to the FITC-labeled Annexin V and stain the cell surface green. During late apoptosis, propidium iodide (PI) enters the cell and stains the contents red. Application 1 Fluorescent imaging of apoptosis induced cells Application 2 Annexin V is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner.

In healthy cells PS is normally only found on the intracellular leaflet of the plasma membrane, but during apoptosis, PS translocates to the external leaflet.